鑫品免疫細胞 -- 鑫品研發實力獲國際期刊「Immunology」肯定
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鑫品研發實力獲國際期刊「Immunology」肯定

Lentiviral calnexin-modified dendritic cells promote expansion of high-avidity effector T cells with central memory phenotype
Bei Wang 1 , Shuhong Han 1 , Lily Lien 2 and Lung-Ji Chang 1
1 Department of Molecular Genetics and Microbiology, University of Florida, College of Medicine, Gainesville, FL, USA, and 2 Vectorite Biomedica Inc., Taipei, Taiwan
 

KEYWORDS
antigen presentation • calnexin • cytotoxic T cells • dendritic cells • immunotherapy

ABSTRACT
Dendritic cells (DCs) are key immune mediators for the education and activation of effector cytotoxic T lymphocytes (CTLs). Ex vivo manipulation of DCs is an attractive strategy in immunotherapy. The chaperone proteins are known to hold the keys to proper protein folding and antigen processing. However, little is known of the role of molecular chaperones in DC and T-cell functions. We report that DCs expressing supraphysiological levels of calnexin, a chaperone protein, via lentiviral gene transfer stimulated the expansion of high-avidity CTLs with increased central memory phenotype. Microarray RNA profiling and analyses of protein expression with flow cytometry and multiplex enzyme-linked immunosorbent assay indicated that calnexin had a global effect on DCs with up-regulation of immune modulatory signals including costimulatory molecules, cytokines, chemokines and adhesion molecules. Compared with unmodified DCs, calnexin-DCs were capable of activating T cells to exhibit increased functional avidity associated with up-regulation of CCR7 and costimulatory tumour necrosis factor receptor superfamily molecules. These findings demonstrate a prominent role of calnexin in optimizing DC immunity with potential for improving immunotherapy.

 

資料來源:Immunology Volume 128 Issue 1,Pages 43-57 
Published Online: 26 Fev 2009 


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